Immunocytochemistry/Immunofluorescence analysis of Integrin beta 1 (CD29) in HeLa cells using ITGB1 Monoclonal Antibody (Product # MA5-38286) diluted at 1:61, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).